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KMID : 0380620190510050503
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 5 p.503 ~ p.508
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Cellular protective effect of Ecklonia cava extract on ultra-fine dust (PM2.5)-induced cytoxicity
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Park Seon-Kyeong
Kang Jin-Yong
Kim Jong-Min
Yoo Seul-Ki
Han Hye-Ju
Shin Eun-Jin
Heo Ho-Jin
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Abstract
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To evaluate the protective effect of Ecklonia cava on ultra-fine dust (PM2.5)-induced cytotoxicity, we investigated the in vitro antioxidant activity and cell viability after exposure to PM2.5. E. cava was extracted using water and 80% ethanol, and antioxidant activity was determined using the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)/2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition assays. The 80% ethanol extract showed relatively higher antioxidant activity than the water extract. The cell protective effects were determined by measuring the intracellular reactive oxygen species (ROS) content and viability of nasal epithelial (RPMI- 2650), lung epithelial (A549), and brain neuroblastoma (MC-IXC) cells. Results showed that the 80% ethanol extract inhibited ROS production more than the water extract. In contrast, both extracts showed similar effects on cell viability in the PM2.5-induced cell death assay. Thus, Ecklonia cava may act as an effective resource for preventing PM2.5-induced cytotoxicity in nasal, lung, and brain cells.
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KEYWORD
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Ecklonia cava, ultra-fine dust, PM2.5, cell protective effect
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